Preparation of biotin–tyramineThe SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Standard curve for Quant-iT OliGreen ssDNA M5 Plate Reader Assay with 200 µl sample volume Quantification of the AAV 8-GFP-CMV viral DNA using the Quant-iT™ OliGreen® ssDNA reagent (Cat. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. The empty LNP matrix solution was made by mixing 300 μL of empty LNP solution with 45 mL diluent. 0. 5. Immunohistochemistry Four μm kidney sections were heated at 65 °C for 1 hr and deparaffinized in xylene, followed by rehydration in decreasing concentrations of ethanol (two washes in 100% ethanol, two washes in 95%, one wash in 70%, one wash in 50%,. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 2. Thesupplyofhumidifiedair (zero air) was controlled by a solenoid valve with on and offLastly, Jurkat cells were least susceptible to Vorinostat treatment (all IC 50 > 50. com 9. Cell Titer Glo reagent (Promega) was then added to each well, and luminescence (RLU) was measured on a SpectraMax M5 plate reader (Molecular Devices). GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. was measured atdetermined450 nm using SpectraMax M5 plate reader. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. d. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. The plate was read by a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm with 620 nm as reference. A solution for low volume applications. After the plate is read, data will appear in the plate. T-test comparisons of group mean values (+/− SEM) were used to determine significance of genotype. Description Figure 1. Appropriately. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 5. OD 600 and luminescence were measured at 30 minute intervals for 48 hours. Help the planet as you cruise along at 5X speed. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Options include top and bottom detection, temperature control and reagent injector. siRNA (750 pmol) was transfected into NRK-49F (1 × 10 6 cells) using a Nucleofector Kit V and the Amaxa Nucleofector. The absorbance was measured using a SpectraMax M5 plate reader from Molecular Divices (San Jose, CA, USA). Dimensions 20" D x 16. After mixing all the components, the absorbance of the mixture was continuously measured at 475 nm at 25°C using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. The TRITC and FITC labeling process is conducted as in a previous study . Thank you!. Product Type. limit of detection (LLD) for ATP was estimated for each microplate reader using theThe relabeled data may be saved for further analysis as either a . 11. Typical settings for fluorescein are 485/520 and. 0 . Fluorescence Microplate Reader Comparison Chart. The assay was performed on HDL collected. Third this recommendation. For a Price List, please call (858) 646-3100 ext. 10569The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. This microplate reader handles Absorbance, Fluorescence Intensity, luminescence, Time resolved fluorescence, fluorescence polarization. The WT DENV2C and its single-point mutants L50S, L54S, L81N and I88N were. 2. Accordingly there are different configurations possible. plates in 150 ll OS minimal medium (Schnider-Keel et al. 4 software (Molecular Devices, San Jose, CA, USA). harvard. Select the lab user profile. Kojic acid was used as a positive control. 5 × 10 5 counts/fmol when measured with a Spectromax M5 plate reader using white opaque 96-well plates. 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. Protein concentration of viral suspensions was measured using a Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific) with a mucin standard curve read on a SpectraMax M5 plate reader (Molecular Devices). View the Molecular Devices website. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. If a more sensitive plate reader is used, the detection sensitivity of Eu 3+ will be about tenfold higher. Fluorescence Microplate Reader Comparison Chart. The percentage of inhibition was calculated using the following formula: O D s R A G E - O D s a m p l e O D s R A G E × 100 ( % ) where OD sRAGE was the signal obtained for sRAGE incubated without the. An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. #655801. 25 For direct lysis of clots by plasmin, plasmin (250nM) was added to the surface of clots and lysis monitored by theSteady-state affinity measurements were performed on an Analyst AD, Analyst HT, or SpectraMax M5 plate reader (Molecular Devices). The plates were washed thrice with kit wash buffer; 150 µl kit substrate was then added to each well and the plate incubated 30 min at room temperature (with shaking). wavelength and 595 nm emission wavelength by Spectramax M5 plate reader (Molecular Devices). Chemistry Department Facilities. The sample is typically placed in a cuvette through which light is sent horizontally. 0 . The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. Fluorescence Microplate Reader Comparison Chart. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. We use the SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). There is currently no charge for using this plate reader. Microplate Reader Shelf. 4. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). 9. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. ". Certain functionalities in the table above requires specific configurations. EPOCH2 Plate Reader/Spectrophotometer. The SpectraMax 190 reader can be integrated with Molecular . 1. Transfection of siRNA into cells. We use Spectramax M5 microplate reader in our lab. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 1, and the color is formed by the reaction in the microtiter plate. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. 130 Watts max. Temperature variation ± 0. Cell Viability Assay Using Violet Staining The cells were seeded into 24-well plates and cultured in normal or growth medium or hormone-depleted medium with the relevant treatments as. The multi-mode. Last lab had ours for years and never had a problem with it. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. If the computer is connected to the Internet: Select Search Automatically for. The plate was covered and mixed on an orbital shaker for five minutes and then placed in a SpectraMax i3x reader and. † Cells in the paper scaffolds and collected in the well plates were also imaged with an Olympus IX-70 fluorescence microscope equipped with a 10×. After cell attachment, culture media was replaced with F-12 media containing 1% FBS for overnight incubation. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 313 reference gain check fail. The SpectraMax iD5 User Guide is a comprehensive document that provides detailed instructions on how to set up, operate, and maintain the SpectraMax iD5 Multi-Mode Microplate Reader. Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readers Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The same procedure. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 3. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. PMC full text: Oncotarget. 99 was required for a valid standard curve. Turn on the SpectraMax M5 Multi-Mode Microplate Reader. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Select Start>Control panel>Device Manager. Activity assays were performed in clear, flat-bottomed 96-well plates and the decrease in absorbance at 340 nm was recorded using a SpectraMax M5 plate reader (Molecular Devices). The measured specific activity of NanoLuc is 1. Reader Documentation Version No. Eis inhibitors were dissolved in Tris-HCl (50 mM, pH 8. That explains the lower values on the LmaxII384 plate reader. Even better, they require MINIMAL modification to. 2013): the specific activity of both enzymes was ~1. The software is simple to use and set up protocols. Most papers they cultured bacteria to OD600>0. SpectraTest ABS2 Absorbance Validation Package performance tests. 2016 Aug 2; 7(31): 50027–50042. 1. 25 pM (0. RNA for the RNA-Seq experiment was obtained exactly as described previously for the quantitative RT-PCR. Fast protein liquid chromatography (FPLC) was performed as the last protein purification step on a Bio-Rad BioLogic DuoFlow using a HighPrep TM 26/60 Sephacryl TM S-200 High Resolution column. Select Update Driver. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. After the plate is read, data will appear in the plate. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. An equal volume of 0. 306 plate air cal fail,low light: 316 grating motor driver faulty: 307 cuv air ref fail: 317 monitor ADC faultyThe BioTek Cytation 5 cell imaging multimode reader combines automated digital widefield microscopy with conventional multimode microplate reading. A. $4,995. Following lysis, the plate was centrifuged at 500g for 3 min and supernatants transferred to a Maxisorp 96-Well Flat-Bottom Assay Plate (Nunc). Enzyme activity from tissue homogenates was normalized to total protein content with the Bradford protein assay (Biorad). 1 M NaCl at varying pH levels (3–10) and incubated at room temperature for 1 h. Lysin A constructs were expressed in M. A solution for low volume applications. The luminescent signal was recorded on a SpectraMax M5 plate reader (Molecular Dy-namics). Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. Release of free AMC by proteolytic activity was monitored on Molecular Devices M5 plate reader with excitation and emission wavelengths of 360 and 460 nm, respectively. 17. SA-β-gal assay. Absorbance readings were then taken at 650 nm using a Spectramax M5 plate reader with SoftMax Pro v5. Junichi Yamamoto a, Takumi Ito b, Yuki Yamaguchi a and Hiroshi Handa * c a School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan b Institute of Medical Science, Tokyo Medical. com 10. Fluorescence Microplate Reader Comparison Chart. The Molecular Devices Spectramax M5 Multi-Mode Plate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. Plate Reader (3) Name Price; EnSpire Flexstation 3 SpectraMax M5 Plate. Wolfe1, and Stephen M. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. For example, microplates containing different samp les can be read using the same orThe 526/470 ratios for the standards were plotted against the standard concentrations. Top reading is usually more sensitive. After 2 h of incubation, the plates were shaken, and the absorbance was measured using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm (OD450). After this time period, the plate was read for fluorescence intensity at ex/em wavelengths of 532/590 nm, on a SpectraMax M5 plate reader. 3. 9. Optimized for fluorescence microplate readers. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 304 signal level saturation. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Specifications General -Detection mode Fluorescence, Luminescence -Read method End point, kinetic. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 2. In the M2, M5 and Plus. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch: Step 4: Incubate at room temperature for 1 hour, protected from light. 0 mM of NADH per min at pH 8. Simply unpack it, plug it in, and begin your experiments. Standards and QCs are composed of a. To eliminate the differences of TCR expression levels among different JRT cell lines, relative luminescence unit (RLU) was normalized to the condition with Human T-Activator DynaBeads at 2:1 bead-to-cell ratio. The Formazan crystals formed in each well were dissolved by adding 100 µl DMSO. Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Plates were incubated at 37°C for 6 hours and then equilibrated to room temperature for 30 min. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Wells with an OD 600[0. Here, we build on this capability and develop an inducible, self‐propagating transposon platform for continuous genome‐wide mutagenesis and the dynamic. We therefore gathered salient. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. 9. The multi-mode. Fluorescence, luminescence, and UV-Vis absorbance multimode plate reading. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86After 5 minutes of incubation at room temperature, luciferase activity was assessed by SpectraMax M5 plate reader (Molecular Probes). 5 hours after an initial 10-minute incubation period. 5. 3. Imagine a world where everyone smiles®. 05 were scored as. 0200-7027. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. RUO. Detection modalities. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 2 only) SpectraMax M-series Microplate readers. 18632/oncotarget. The plate was incubated for 4 h and the absorbance of the solubilized formazan was measured on a Molecular Devices Spectramax M5 plate reader at 570 nm. Select the Driver tab. Cells were collected and fixed with precooled 95 % ethanol at 4 ºC for 12 h. The solution was shaken at 37°C and 1200 rpm for 3 days. Power on the Epoch Biotek Gen 5 absorbance microplate reader. Page 1 of 8 . The Infinite and Spark multimode microplate readers are modular and upgradeable. The password is. 7°C (gradient heating design). The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Turn the reader on and secure the USB cable. Highly selective for dsDNA over ssDNA or RNA. Open the program, and set the temperature to 37 °C for preheating. 0 and room temperature. Documents. 4: Test tube cover. Flow cytometry. The plates are optimized for fluorescence and luminescence in cell culture and microscopic applications. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. 40266 Email: [email protected]) at room temperature. This user guide provides safety information, warnings, cautions, tips, and instructions for operating the instrument, connecting cables, setting up the instrument, and troubleshooting. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Thank you!. After mixing with freshly diluted substrate (50 μl well −1), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) under luminescence mode. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. doi: 10. Description Figure 1. STAKMAX. 2. Then, luminescence of samples was read in a Spectramax M5 plate reader with integration time of 5 s. Uncontrolled Copy for Training and Reference Purposes Only . 100 μl of 2% EB (Sigma-Aldrich, St. Quantitative PCR RNA was collected in duplicate from 1 × 10 6 cells lysed in TRIzol Reagent (Invitrogen, 15596018) after. The standard PEG assay required larger volumes and thus higher amounts of protein (∼5X) compared to the micro. 25:1 effector to target cells, and the plates were incubated for an additional 3 hours. 40266 Email: drugdiscoverytech@lifetech. The guide also covers the software features, the specifications, and the accessories of the systems. Select Start>Control panel>Device Manager. IMAP assays are based on binding of. The measured bioluminescence data were expressed as mean ± SE (n = 3) and fitted with one-site binding model using the. Five preferred detection modes. Clear Filters. 1. 4: Test tube cover. 91 cm x 44. After the plate is read, data will appear in the plate section: 11. edu, 814-863-3503. U87 and U251 cells were seeded in 96-well plates at 3 × 10 3. The name is the same, but the attitude is completely different. The Chemistry Department is housed in an air-conditioned building, sharing space with the Departments of Biology and Molecular Biology & Biochemistry. Representative fluorescence images of the cell-containing scaffolds and well plate are shown in Fig. Plates were shaken continuously at medium speed, and temperature was kept at 37°C. CD spectra were recorded using a Jasco-715 circular dichroism spectrophotometer at 20 °C. During the room temperature incubation (step 9. To the OP, if you are familiar with VAG-COM, you know that RS232-based VAG-COM cables are in the $14 range on eBay. The IC50 was calculated using the DRC package in the R software program (v3. 1) Select the correct plate and the wells that you want to measure. 13. Copia stocks a full line of used microplate readers. NanoLuc substrate and R3/I5-SmBiT were added to the homogenate of HEK293T cells transiently overexpressing sLgBiT-fused receptor and bioluminescence was measured on a SpectraMax M5 plate reader with an interval of 30 s. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. When the user applies a set temperature of 37°C, the top is heated to 37. I’m. The data analysis was performed by GraphPad Prism. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. both cuvette-based and microplate reader applications on the same instrument. cerevisiae transcription factor Azf1p Taylor Stewart1, Benjamin E. The percent relative binding potency was calculated by dividing the reference material. AC16 cells were treated with control, 5 mmol/L LiCl or 2. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. 1. Wide range of applications. Choose the Experiments icon. Developed with Promega reagents to provide a. Incubation. In my opinion one the best plate readers which I used till now is Molecular Devices SpectraMax M5 Plate reader. Flock Safety is an Atlanta-based company that sells automatic license plate detection readers, or ALPRs for short, to law enforcement agencies, schools, neighborhood homeowners associations and. Fluorescence was measured using microplate reader at an excitation wavelength of 488 nm and emission wavelength of 529 nm using Spectramax-M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). Find regional support. The guide also covers the features, specifications, and applications of the reader, as well as troubleshooting tips and technical support information. The plate reader can measure multiple readouts, including UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. In the M2, M5 and Plus 384, the light. 3). 2. Add 100 μL of sample media and standards (0, 1, 2, 4, 8, 16, 32, 64, 128 μM NaNO 2) to plate in duplicate. 40266 Email: [email protected]. ScanLater Western Blot Detection Cartridge. Lastly, Jurkat cells were least susceptible to Vorinostat treatment (all IC 50 > 50. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. There is currently no charge for using this plate reader. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Models for In. Information on how to reserve the plate readerMolecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. BioTek Gen5 software is an integrated tool for endpoint, kinetic, spectral scanning, and well area scanning applications in a variety of microplates and other labware. Blue, kinase (no inhibitor); green, no kinase. There is currently no charge for using this plate reader. High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities. The exact concentration of the RNA stock solution was calculated by UV absorption at 260 nm (measured by Spectramax M5 Plate Reader, Molecular Devices, San Jose, CA) with the accepted extinction coefficient of the RNA (21. The fluorescent intensity of the cells reflecting the LIP was quantified by measuring the fluorescent signal at excitation wavelength (λex) of 543 nm and emission wavelength (λem) of 580 nm as per the manufacturer recommendation using a SpectraMax M5 plate reader (Molecular Devices) and normalized to the cell count. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 40266 Email: [email protected] standard spectrophotometer measures the absorbance of one sample at a time. StakMax Handling Stacker Base Unit. PURPOSE. Application Area: Multi mode plate reader. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. It controls all the plate reader functions and offers powerful data analysis. 2. Reactions on 384-well plates (Corning) were monitored by fluorescence (emission 545 nm) using a SpectraMax M5 plate reader (Molecular Devices), with excitation at 274 nm, for 20 min at 2-min. Fluorescein 0. were read using SpectraMax M5 plate reader (Molecular Devices, San Jose, CA, USA) with excitation of 380 nm and emission of 460 nm. siRNA oligonucleotides targeted specifically to rat P2X 7 were used in this experiment. 2. Fluorescence was recorded in a Spectramax M5 plate reader at a 0. The degradation. Reader Documentation Version No. We carry both single-mode and multi-mode readers from manufacturers such as BioTek Instruments, Perkin Elmer, and SRU Biosystems. Abs). Protocol for Qubit DNA HS Assay. 2. Users can choose to process all plates within the magazine or select a specific number. 5. 5°C at 37°C. The plate can be shaken (icon right of thermometer, and see automix option in setup). After that, DCFH-DA was used to mark cells and the ROS level was determined by Spectramax M5 plate reader and five parallel samples were prepared for each group (Wu et al. After addition of the freshly diluted substrate (50 μL/well, from Promega), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices) using the luminescence mode. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Bioluminescence assays showed that the engineered Luc-Cys was fully active when compared with 6 × His-NanoLuc prepared in our previous work (Zhang et al. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. When a feature or capability applies to only certain readers, this. 1. 8K members Since 2000 M5Board is the best forum community for information on the BMW M5 E60 (V-10), E39 (V-8), E34. 2. Molecular Devices. 51 L/g). Then, absorbance was measured by a Spectra Max ™ M5 plate reader (Molecular Devices, Sunnyvale, CA). The dual monochromator optics allow. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Activation of reporter constructs was monitored using the Dual-Glo Luciferase assay (Promega) and measured in a Spectramax M5 plate reader. Finally, the absorbance of color development was read by SpectraMax M5 plate reader (Molecular Devices, San Jose, USA) at wavelength of 450 nm. ZERO BIAS - scores, article reviews, protocol conditions and more. Purified fluorescent protein was diluted 15-fold into 0.